Lee Y H, Bae S S, Seo J K, Choi I, Ryu S H, Suh P G
Department of Life Science and School of Environmental Engineering, Pohang University of Science and Technology, Korea.
Mol Cells. 2000 Aug 31;10(4):469-74.
Phospholipase C (PLC)-gamma1 plays a pivotal role in the signal transduction pathway mediated by growth factors. In this study, we found that neurite outgrowth of pheochromocytoma (PC12) cells was significantly induced by interleukin-6 (IL-6). Stimulation of PC12 cells with IL-6 led to tyrosine phosphorylation of PLC-gamma1 in a dose- and time-dependent manner. IL-6 stimulation also increased the hydrolysis of phosphatidylinositol 4,5-bisphosphate. Accumulation of total inositol phosphate as well as tyrosine phosphorylation of PLC-gamma1 was inhibited by the pretreatment of protein kinase inhibitors such as genistein and staurosporine. These results suggest that PLC-gamma1 may be involved in the signal transduction pathway of IL-6-induced PC12 cell differentiation.
磷脂酶C（PLC）-γ1在生长因子介导的信号转导途径中起关键作用。在本研究中，我们发现白细胞介素-6（IL-6）能显著诱导嗜铬细胞瘤（PC12）细胞的神经突生长。用IL-6刺激PC12细胞会导致PLC-γ1的酪氨酸磷酸化呈剂量和时间依赖性。IL-6刺激还增加了磷脂酰肌醇4,5-二磷酸的水解。蛋白激酶抑制剂如染料木黄酮和星形孢菌素的预处理可抑制总肌醇磷酸的积累以及PLC-γ1的酪氨酸磷酸化。这些结果表明，PLC-γ1可能参与IL-6诱导的PC12细胞分化的信号转导途径。
