GLI1的转录活性受到蛋白激酶A的负调控。

Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

作者信息

Kaesler S, Lüscher B, Rüther U

机构信息

Institut für Molekularbiologie, Medizinische Hochschule Hannover, Germany.

出版信息

Biol Chem. 2000 Jul;381(7):545-51. doi: 10.1515/BC.2000.070.

DOI:10.1515/BC.2000.070

PMID:10987360

Abstract

The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence for proteolytic processing or for alteration in the subcellular distribution of GLI1 was obtained. The responsive PKA site (aa333-336) was localized to the second zinc finger of GLI1. Mutation of Ser336 revealed that PKA could also stimulate GLI1 transcriptional activity. Thus, our data demonstrate both negative and positive regulation of human GLI1 by PKA.

摘要

转录因子GLI1是GLI家族三个脊椎动物成员之一，其特征是具有由五个锌指组成的高度保守的DNA结合结构域。我们分析了人类GLI1是否是蛋白激酶A（PKA）调控的靶点。结果发现PKA抑制GLI1的转录活性。然而，未获得GLI1蛋白水解加工或亚细胞分布改变的证据。PKA反应位点（氨基酸333 - 336）定位于GLI1的第二个锌指。丝氨酸336的突变表明PKA也可以刺激GLI1的转录活性。因此，我们的数据证明了PKA对人类GLI1具有负向和正向调控作用。