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Genes Dev. 2000 Mar 1;14(5):559-73.
2
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Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green algae.紫菜线粒体DNA的完整序列。蓝藻内含子以及红藻和绿藻的共同祖先。
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Biochemistry. 1999 Mar 9;38(10):3157-67. doi: 10.1021/bi982462j.
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Tight binding of the 5' exon to domain I of a group II self-splicing intron requires completion of the intron active site.5'外显子与II组自我剪接内含子的结构域I紧密结合需要内含子活性位点的形成。
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Differential chemical probing of a group II self-splicing intron identifies bases involved in tertiary interactions and supports an alternative secondary structure model of domain V.对II组自剪接内含子进行差异化学探测，确定了参与三级相互作用的碱基，并支持结构域V的另一种二级结构模型。
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DNA strand breaking by the hydroxyl radical is governed by the accessible surface areas of the hydrogen atoms of the DNA backbone.羟基自由基导致的DNA链断裂由DNA主链氢原子的可及表面积决定。
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对II类自剪接内含子外显子结合的三维视角。

A three-dimensional perspective on exon binding by a group II self-splicing intron.

作者信息

Costa M, Michel F, Westhof E

机构信息

Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA.

出版信息

EMBO J. 2000 Sep 15;19(18):5007-18. doi: 10.1093/emboj/19.18.5007.

DOI:10.1093/emboj/19.18.5007
PMID:10990464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC314214/
Abstract

We have used chemical footprinting, kinetic dissection of reactions and comparative sequence analysis to show that in self-splicing introns belonging to subgroup IIB, the sites that bind the 5' and 3' exons are connected to one another by tertiary interactions. This unanticipated arrangement, which contrasts with the direct covalent linkage that prevails in the other major subdivision of group II (subgroup IIA), results in a unique three-dimensional architecture for the complex between the exons, their binding sites and intron domain V. A key feature of the modeled complex is the presence of several close contacts between domain V and one of the intron-exon pairings. These contacts, whose existence is supported by hydroxyl radical footprinting, provide a structural framework for the known role of domain V in catalysis and its recently demonstrated involvement in binding of the 5' exon.

摘要

我们运用化学足迹法、反应动力学剖析以及比较序列分析，以表明在属于IIB亚组的自我剪接内含子中，结合5'和3'外显子的位点通过三级相互作用彼此相连。这种意外的排列方式与II组另一主要细分（IIA亚组）中普遍存在的直接共价连接形成对比，导致外显子、它们的结合位点与内含子结构域V之间的复合物具有独特的三维结构。模拟复合物的一个关键特征是结构域V与其中一个内含子 - 外显子配对之间存在几个紧密接触。这些接触得到了羟基自由基足迹法的支持，为结构域V在催化中的已知作用及其最近证明的参与5'外显子结合提供了结构框架。