Vanderplasschen A
Bull Mem Acad R Med Belg. 1999;154(6 Pt 2):363-9.
This work was devoted to the study of the interactions between bovine herpesvirus 4 (BHV-4) and bovine cells in vitro. It led to the discovery of two interesting properties of BVH-4 replication cycle: first, the cellular receptor heparan sulfate was proven to mediate BVH-4 binding to target cells. This is the first description of the implication of heparan sulfate in the binding process of a gammaherpesvirus. Second, using synchronised cells, the replication of BVH-4 DNA was proven to be dependent on the S phase of the cell cycle. This dependence could explain some properties of BVH-4 infection in vitro and could play an important role in the biology of the infection in vivo. Finally, in order to produce monoclonal antibodies against BVH-4 IE1 and IE2 proteins, the genes coding for these proteins were cloned and expressed in prokaryotic cells.
这项工作致力于研究牛疱疹病毒4（BHV - 4）与牛细胞在体外的相互作用。它导致发现了BHV - 4复制周期的两个有趣特性：第一，细胞受体硫酸乙酰肝素被证明介导BHV - 4与靶细胞的结合。这是首次描述硫酸乙酰肝素在γ疱疹病毒结合过程中的作用。第二，使用同步化细胞，证明BHV - 4 DNA的复制依赖于细胞周期的S期。这种依赖性可以解释BHV - 4体外感染的一些特性，并且可能在体内感染生物学中起重要作用。最后，为了产生针对BHV - 4 IE1和IE2蛋白的单克隆抗体，编码这些蛋白的基因被克隆并在原核细胞中表达。
