Deinard A S, Kidd K K
Department of Anthropology, Yale University, New Haven, CT 06520, USA.
DNA Seq. 1998;8(5):289-301. doi: 10.3109/10425179809034074.
Although direct DNA sequencing may allow rapid and high quality comparative phylogenetic analyses among species, such an approach may not be the most efficient method by which to make a large number of cross-species comparisons. We illustrate the use of Denaturing Gradient Gel Electrophoresis (DGGE) to screen a D2 Dopamine Receptor intron for DNA sequence variation, both within and between closely related species, in order to infer their evolutionary relationships. Our results suggest that: a) humans have less genetic variation than the great apes; b) pygmy chimpanzees have less genetic variation than common chimpanzees; and c) DNA sequence comparative analyses of primates require adequate sampling, both in number and in geographical range.
虽然直接DNA测序可能允许在物种间进行快速且高质量的比较系统发育分析，但这种方法可能不是进行大量跨物种比较的最有效方法。我们举例说明了使用变性梯度凝胶电泳（DGGE）来筛选D2多巴胺受体内含子，以检测密切相关物种内部和之间的DNA序列变异，从而推断它们的进化关系。我们的结果表明：a）人类的遗传变异比大猩猩少；b）侏儒黑猩猩的遗传变异比普通黑猩猩少；c）灵长类动物的DNA序列比较分析需要在数量和地理范围上进行充分的抽样。
