Ostashevsky J Y
Department of Radiation Oncology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA.
Int J Radiat Biol. 2000 Sep;76(9):1179-87. doi: 10.1080/09553000050134410.
To discuss the effects of chromosome structure on the inter- and intrachromosomal exchanges in the framework of the model, which argues that the higher-order chromosome structure contains 10-30 Mbp loop clusters (micelles).
Intermicelle contacts determine exchange-type chromosome aberrations. Ratios of inter- to intrachromosomal exchanges calculated in the model are similar to the experimental data (literature) for human lymphocytes and flat fibroblasts. The frequency of interchanges is affected by nuclear shape; this might explain the greater number of interchanges observed in 3-D spherical lymphocytes vs. that in 2-D flat fibroblasts. Chromosome configuration (linear vs. folded Rab1) affects the pattern of micelle contacts. The model predicts that chromosomes in haploid Tradescantia microspores have the folded Rab1 orientation; this explains quantitatively the low value of the ratio of dicentrics to centric rings observed in these cells.
在该模型框架内讨论染色体结构对染色体间和染色体内交换的影响，该模型认为高阶染色体结构包含10 - 30兆碱基对的环簇（微团）。
微团间接触决定交换型染色体畸变。该模型计算出的染色体间与染色体内交换比率与人类淋巴细胞和平坦成纤维细胞的实验数据（文献）相似。互换频率受细胞核形状影响；这可能解释了在三维球形淋巴细胞中观察到的互换数量比二维平坦成纤维细胞中更多的现象。染色体构型（线性与折叠的Rab1）影响微团接触模式。该模型预测单倍体紫露草小孢子中的染色体具有折叠的Rab1方向；这定量解释了在这些细胞中观察到的双着丝粒与着丝粒环比率的低值。
