烟酰胺腺嘌呤二核苷酸（NADH）和过氧化氢（H₂O₂）对铬酸盐诱导的人红细胞血红蛋白氧化和过氧化的影响。

Effects of NADH and H(2)O(2) on chromate-induced human erythrocytes hemoglobin oxidation and peroxidation.

作者信息

Fernandes M A, Geraldes C F, Oliveira C R, Alpoim M C

机构信息

Departamento de Zoologia, Universidade de Coimbra, Coimbra, 3004-517, Portugal.

出版信息

Ecotoxicol Environ Saf. 2000 Sep;47(1):39-42. doi: 10.1006/eesa.1999.1939.

DOI:10.1006/eesa.1999.1939

PMID:10993701

Abstract

The effects of NADH and H(2)O(2) on chromate-induced human erythrocyte hemoglobin oxidation and peroxidation were studied. It was observed that NADH decreases the levels of chromate-induced human erythrocyte hemoglobin oxidation and peroxidation. H(2)O(2) decreases the levels of chromate-induced hemoglobin oxidation, but increases the levels of chromate-induced peroxidation. The ability of H(2)O(2) to decrease the levels of chromate-induced hemoglobin oxidation is higher than that observed for NADH. Furthermore, H(2)O(2) increases the inhibitory effect of NADH on chromate-induced hemoglobin oxidation, but decreases the NADH effect on chromate-induced peroxidation. The meaning of these results is discussed in terms of involvement of reactive chromium(V) species and reactive oxygen species in the mechanism by which chromate induces its effects in human erythrocytes.

摘要

研究了NADH和H₂O₂对铬酸盐诱导的人红细胞血红蛋白氧化和过氧化的影响。观察到NADH可降低铬酸盐诱导的人红细胞血红蛋白氧化和过氧化水平。H₂O₂可降低铬酸盐诱导的血红蛋白氧化水平，但会增加铬酸盐诱导的过氧化水平。H₂O₂降低铬酸盐诱导的血红蛋白氧化水平的能力高于NADH。此外，H₂O₂增强了NADH对铬酸盐诱导的血红蛋白氧化的抑制作用，但降低了NADH对铬酸盐诱导的过氧化的作用。从活性铬（V）物种和活性氧物种参与铬酸盐在人红细胞中诱导其效应的机制方面讨论了这些结果的意义。