Zakharova N S, Petrova T A
Bach Institute of Biochemistry of the Russian Academy of Sciences, Moscow, Russia.
Prikl Biokhim Mikrobiol. 2000 Jul-Aug;36(4):458-61.
beta-Glucosidases detected in the leaves and roots of common beet, Beta vulgaris, have been demonstrated to catalyze hydrolysis of native betacyanins. A method is described for the isolation and purification of beta-glucosidase from the roots, which involves ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex gel filtration. Maximum activity of the enzyme is detected at 50 degrees C and pH 8.0; it retains stability within the pH range from 5.1 to 9.2. In the leaves, beta-glucosidase is chloroplast membrane-associated; solubilization of the membranes results in the enzyme inactivation.
在普通甜菜（Beta vulgaris）的叶片和根部检测到的β-葡萄糖苷酶已被证明可催化天然甜菜色素苷的水解。本文描述了一种从根部分离和纯化β-葡萄糖苷酶的方法，该方法包括硫酸铵沉淀、DEAE-纤维素色谱和葡聚糖凝胶过滤。该酶在50℃和pH 8.0时检测到最大活性；在pH 5.1至9.2范围内保持稳定。在叶片中，β-葡萄糖苷酶与叶绿体膜相关；膜的溶解导致酶失活。
