Conversion of glutamate to glutamine by permeabilized Corynebacterium glutamicum.

Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing glutamate as the sole source of nitrogen.