Behnke B, Johansson J, Bayer E, Nilsson S
BASF AG, Agrarzentrum Spectroskopie APD/CS, Limburgerhof, Germany.
Electrophoresis. 2000 Sep;21(15):3102-8. doi: 10.1002/1522-2683(20000901)21:15<3102::AID-ELPS3102>3.0.CO;2-V.
The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase packing material were studied as well as at the transition from stationary phase to the outlet frit and the open tubular area. Striking differences in signal intensity and separation efficiency were revealed depending on where on the column detection was performed. Furthermore, adsorption of the analytes on the frits was observed.
采用基于激光诱导荧光成像的检测方法，对常用于毛细管液相色谱（LC）或毛细管电色谱（CEC）的毛细管分离系统中5种丹磺酰化氨基酸的梯度进行了跟踪，并进行了详细研究。检测系统由XeCl准分子激光器和图像增强型电荷耦合器件（CCD）相机组成。沿毛细管柱的荧光强度分布图在计算机屏幕上显示并不断更新。该检测系统能够监测柱内的分离动力学。实验重点关注事件的过程，特别是在界面处。研究了反相填充材料开始时以及从固定相到出口烧结板和开放管区域过渡时发生的过程。根据在柱上进行检测的位置，发现信号强度和分离效率存在显著差异。此外，还观察到分析物在烧结板上的吸附。
