聚半乳糖醛酸寡聚物的电泳研究及其与金属离子的相互作用。
Electrophoretic studies of polygalacturonate oligomers and their interactions with metal ions.
作者信息
Wiedmer S K, Cassely A, Hong M, Novotny M V, Riekkola M L
机构信息
Department of Chemistry, Indiana University, Bloomington 47405, USA.
出版信息
Electrophoresis. 2000 Sep;21(15):3212-9. doi: 10.1002/1522-2683(20000901)21:15<3212::AID-ELPS3212>3.0.CO;2-N.
Polygalacturonic acid, a linear homopolysaccharide, was investigated by capillary electrophoresis (CE) using linear polyacrylamide-coated capillaries and laser-induced fluorescence (LIF) detection. A successful separation of its fluorescently labeled oligomers was achieved through sieving in polyacrylamide entangled matrices. The reaction conditions for the derivatization of polygalacturonic acid were optimized. In studying the interactions between polygalacturonic acid and various metal ions, the end-label, free-solution electrophoretic (ELFSE) technique, developed earlier in our laboratory (Sudor, J., Novotny, M. V., Anal. Chem. 1995, 67, 4205-4209) was found preferable to the sieving method. ELFSE is fast and convenient in that no polymer solutions are needed for the separation. The investigation showed that for the moderately large oligomers, the strongest binding occurred with calcium and cadmium ions, while the smallest interaction was observed with magnesium ions.
聚半乳糖醛酸是一种线性同多糖，采用线性聚丙烯酰胺涂层毛细管和激光诱导荧光（LIF）检测技术，通过毛细管电泳（CE）对其进行了研究。通过在聚丙烯酰胺缠结基质中筛分，成功分离了其荧光标记的低聚物。优化了聚半乳糖醛酸衍生化的反应条件。在研究聚半乳糖醛酸与各种金属离子之间的相互作用时，发现我们实验室早期开发的末端标记、自由溶液电泳（ELFSE）技术（Sudor，J.，Novotny，M. V.，Anal. Chem. 1995，67，4205 - 4209）比筛分方法更可取。ELFSE快速便捷，因为分离过程中不需要聚合物溶液。研究表明，对于中等大小的低聚物，与钙离子和镉离子的结合最强，而与镁离子的相互作用最小。
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