评估霉菌及其过敏原暴露情况的标志物抗原。I. 烟曲霉。
Marker antigens to assess exposure to molds and their allergens. I. Aspergillus fumigatus.
作者信息
Wijnands L M, Deisz W D, van Leusden F M
机构信息
Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
出版信息
Allergy. 2000 Sep;55(9):850-5.
BACKGROUND
Exposure to molds and mold products in the indoor environment may lead to allergies, asthma, or respiratory complaints in general. Enumeration of viable parts of molds in the environment is insufficient to estimate exposure. Therefore, other methods have to be developed.
METHODS
Aspergillus fumigatus (Af) was grown under various circumstances (22 degrees C and 30 degrees C, high and low water activity) in the laboratory. At various moments during culture, extracts were taken, and antigen and allergen content was examined by acrylamide electrophoresis and immunoblot.
RESULTS
In extracts of the culture filtrate, two antigens were found to be produced under all studied growth conditions (common antigens). In the extracts of the water-soluble portion of the mycelium, one common antigen was found.
CONCLUSIONS
The three common antigens may serve as marker antigens for exposure to Af and its products. In view of the simultaneous presence of two of these common antigens with Af allergens, these two marker antigens may be used to estimate exposure to allergens of Af.
背景
在室内环境中接触霉菌及其产物可能会引发过敏、哮喘或一般的呼吸道疾病。对环境中霉菌的存活部分进行计数不足以估计接触情况。因此，必须开发其他方法。
方法
在实验室中，烟曲霉（Af）在各种条件下（22摄氏度和30摄氏度，高水分活度和低水分活度）生长。在培养过程中的不同时间点提取提取物，并通过丙烯酰胺电泳和免疫印迹检测抗原和过敏原含量。
结果
在培养滤液的提取物中，发现在所有研究的生长条件下都会产生两种抗原（共同抗原）。在菌丝体水溶性部分的提取物中，发现了一种共同抗原。
结论
这三种共同抗原可作为接触烟曲霉及其产物的标记抗原。鉴于其中两种共同抗原与烟曲霉过敏原同时存在，这两种标记抗原可用于估计对烟曲霉过敏原的接触情况。
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