在分化的嗜酸性粒细胞上功能性β7整合素的选择性上调。
Selective upregulation of a functional beta7 integrin on differentiating eosinophils.
作者信息
Lundahl J, Sehmi R, Hayes L, Howie K, Denburg J A
机构信息
Department of Medicine, McMaster University, Hamilton, ON, Canada.
出版信息
Allergy. 2000 Sep;55(9):865-72. doi: 10.1034/j.1398-9995.2000.00574.x.
BACKGROUND
The sequence of adhesion-molecule expression during eosinophil differentiation remains unclear.
METHODS
We analyzed the surface expression of alpha4, beta1, and beta7 integrins and compared it to established myeloid developmental markers, using the eosinophilic cell line HL-60 clone 15, as well as cord and peripheral blood differentiation assays.
RESULTS
Cells induced to eosinophil differentiation by treatment with butyric acid, IL-5, and GM-CSF showed a significant upregulation of beta7 integrin expression coincident with a marked upregulation of CD35 and attenuation of CD33 and beta1 integrin expression. In addition, adhesion of induced HL-60 clone 15 cells to fibronectin was attenuated by a beta7 integrin antibody.
CONCLUSIONS
Our data show that protein synthesis-dependent upregulation of the functional beta7 integrin occurs under conditions when beta4 and beta1 integrins are fully expressed, indicating a sequential appearance of specific adhesion molecules on differentiating eosinophil progenitors.
背景
嗜酸性粒细胞分化过程中黏附分子表达的顺序仍不清楚。
方法
我们使用嗜酸性细胞系HL-60克隆15以及脐带血和外周血分化试验，分析了α4、β1和β7整合素的表面表达，并将其与已确定的髓系发育标志物进行比较。
结果
用丁酸、白细胞介素-5和粒细胞-巨噬细胞集落刺激因子处理诱导嗜酸性粒细胞分化的细胞，显示β7整合素表达显著上调，同时CD35明显上调，CD33和β1整合素表达减弱。此外，β7整合素抗体可减弱诱导的HL-60克隆15细胞与纤连蛋白的黏附。
结论
我们的数据表明，在β4和β1整合素充分表达的条件下，功能性β7整合素会发生蛋白质合成依赖性上调，这表明在分化的嗜酸性粒细胞祖细胞上特定黏附分子会依次出现。
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