Camarero S, Ruiz-Dueñas F J, Sarkar S, Martínez M J, Martínez A T
Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006, Madrid, Spain.
FEMS Microbiol Lett. 2000 Oct 1;191(1):37-43. doi: 10.1111/j.1574-6968.2000.tb09316.x.
We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn(2+) and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.
我们报道了从白腐真菌刺芹侧耳的木质纤维素培养物中分离出的编码一种多功能过氧化物酶的基因ps1的克隆和测序，该酶兼具木质素过氧化物酶（LiP）和锰过氧化物酶（MnP）的催化特性。该基因含有15个推定的内含子，推导的氨基酸序列由一个339个残基的成熟蛋白和一个31个残基的信号肽组成。在启动子区域鉴定出了几个推定的反应元件。通过与黄孢原毛平革菌的MnP和LiP分别类推预测，参与通过直接电子转移到血红素氧化Mn(2+)和芳香族底物以及从表面残基进行长程电子转移的氨基酸残基。给出了一个树状图，说明了29种真菌过氧化物酶之间的序列关系。
