天蓝色链霉菌M145染色体中心附近DNA扩增的鉴定。

Identification of DNA amplifications near the center of the Streptomyces coelicolor M145 chromosome.

作者信息

Redenbach M, Kleinert E, Stoll A

机构信息

Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663, Kaiserslautern, Germany.

出版信息

FEMS Microbiol Lett. 2000 Oct 1;191(1):123-9. doi: 10.1111/j.1574-6968.2000.tb09328.x.

DOI:10.1111/j.1574-6968.2000.tb09328.x

PMID:11004409

Abstract

Linear streptomycete chromosomes frequently undergo spontaneous gross DNA rearrangements at the terminal regions. Large DNA deletions of the chromosome ends are in many cases associated with tandemly reiterated DNA amplifications, found at the border of the deletable areas. In contrast to previous reports, we have discovered amplifications near the center of the Streptomyces coelicolor M145 chromosome. The detected amplified units of DNA are 19.9 kb and 16 kb in length and exist in copy numbers of 30 and 40, respectively. Both amplifications were located in the same region and share at least 3.6 kb.

摘要

线性链霉菌染色体经常在末端区域发生自发的大规模DNA重排。在许多情况下，染色体末端的大片段DNA缺失与串联重复的DNA扩增有关，这些扩增出现在可缺失区域的边界。与之前的报道不同，我们在天蓝色链霉菌M145染色体的中心附近发现了扩增现象。检测到的DNA扩增单元长度分别为19.9 kb和16 kb，拷贝数分别为30和40。这两种扩增都位于同一区域，且至少共享3.6 kb。