Onishi T, Ooshima T, Sobue S, El-Sharaby A, Kurisu K, Wakisaka S
Department of Oral Anatomy and Development Biology, Osaka University Faculty of Dentistry, Suita, Japan.
J Periodontal Res. 2000 Oct;35(5):301-9. doi: 10.1034/j.1600-0765.2000.035005301.x.
The present immunohistochemical study was designed to investigate the alteration in the expression level of calbindin D28k in the periodontal ligament of the rat molar in response to changes in occlusal force to clarify the physiological role(s) of this protein in the ligament. In normal periodontal ligament of the lower first molar, immunoreactivity for calbindin D28k was found in the spindle-shaped cells, presumably fibroblasts, at the alveolar portion of the ligament at the distal side of the mesial root and mesial side of the distal root. Following the overload of occlusal force to the upper first molar by bite-raising, the number and immunoreactivity of the positive cells in the periodontal ligament of the lower first molar increased gradually. A more significant increase was detected at 7 d following the bite-raising compared to the normal animals. When occlusal force was removed by the extraction of the upper first molar, the expression level of calbindin D28k in the periodontal ligament of the lower first molar rapidly decreased, however a subsequent gradual increase was recognized. Statistical analysis of the spatial immunoreactivity of calbindin D28k in the periodontal ligament was performed and showed statistically significant differences. The present results suggest that calbindin D28k may play important roles in the homeostasis and cytoprotection of the periodontal fibroblasts against occlusal force.
本免疫组织化学研究旨在调查大鼠磨牙牙周膜中钙结合蛋白D28k表达水平随咬合力变化的改变情况，以阐明该蛋白在牙周膜中的生理作用。在下颌第一磨牙的正常牙周膜中，在近中根远侧和远中根近侧牙周膜牙槽部分的梭形细胞（可能是成纤维细胞）中发现了钙结合蛋白D28k的免疫反应性。通过垫高咬合使上颌第一磨牙承受咬合力过载后，下颌第一磨牙牙周膜中阳性细胞的数量和免疫反应性逐渐增加。与正常动物相比，垫高咬合后7天检测到更显著的增加。当通过拔除上颌第一磨牙去除咬合力时，下颌第一磨牙牙周膜中钙结合蛋白D28k的表达水平迅速下降，但随后出现逐渐增加。对牙周膜中钙结合蛋白D28k的空间免疫反应性进行统计分析，结果显示具有统计学意义的差异。目前的结果表明，钙结合蛋白D28k可能在牙周成纤维细胞对抗咬合力的稳态和细胞保护中发挥重要作用。
