猫血浆和毛发中特比萘芬测定方法的建立。

The development of the method for the determination of terbinafine in cat's plasma and hair.

作者信息

Kozuh Erzen N, Kuzner J, Drobnic-Kossorok M

机构信息

Institute of Physiology, Pharmacology and Toxicology, Veterinary Faculty, University of Ljubljana, Slovenia.

出版信息

Pflugers Arch. 2000;440(5 Suppl):R168-70.

PMID:11005657

Abstract

Clinical investigations of terbinafine indicate its high treatment activity against infections by several dermatophytes. Its efficiency was tested also in the treatment of microsporosis in cats. The distribution of terbinafine in cat's plasma and hair is important for the identification of the drug efficiency. A fast and reliable reversed-phase high performance liquid chromatographic method with appropriate sample preparation has been developed. Reliability, good reproducibility and low detection limit (LOD 0.25 ng/ml) of the method enable determination of terbinafine in hair and also in plasma of cats infected with Microsporum canis treated by Lamisil tablets.

摘要

特比萘芬的临床研究表明，它对多种皮肤癣菌感染具有较高的治疗活性。它在猫小孢子菌病治疗中的有效性也得到了测试。特比萘芬在猫血浆和毛发中的分布对于确定药物疗效很重要。已开发出一种采用适当样品制备方法的快速可靠的反相高效液相色谱法。该方法的可靠性、良好的重现性和低检测限（检测限为0.25 ng/ml）能够测定感染犬小孢子菌并接受兰美抒片治疗的猫毛发及血浆中的特比萘芬。