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固有层对体外尿路上皮细胞生长和分化的影响。

The effect of lamina propria on the growth and differentiation of urothelial cells in vitro.

作者信息

Erdani Kreft M, Sterle M

机构信息

Institute of Cell Biology, Medical Faculty, Ljubljana, Slovenia.

出版信息

Pflugers Arch. 2000;440(5 Suppl):R181-2.

Abstract

The effect of lamina propria on the growth and differentiation of the mouse urinary bladder urothelial cells in vitro has been studied by light and electron microscopy using morphological and immunohistochemical methods. Three different types of urothelial cultures were maintained on a porous membrane in serum-free medium for 10 days. Our results showed that urothelial cells in culture types I and II were organised as the multilayer epithelium showing terminal differentiation, from the porous membrane toward the surface. On the surface of culture type I and II, superficial urothelial cells were highly differentiated. They formed tight junctions and had plaques of an asymmetric unit membrane, a hallmark of terminal differentiation in bladder superficial cells. These cultured superficial cells were also cytokeratin 20 positive. On the contrary, urothelial cells in culture type III have shown a limited capacity for survival and have grown as poorly differentiated monolayer. These results revealed that reciprocal intercellular signalling between the lamina propria and urothelial cells is essential to achieve terminal differentiation of urothelial cells in vitro.

摘要

利用形态学和免疫组织化学方法,通过光学显微镜和电子显微镜研究了固有层对体外培养的小鼠膀胱尿路上皮细胞生长和分化的影响。在无血清培养基中,将三种不同类型的尿路上皮培养物置于多孔膜上培养10天。我们的结果表明,I型和II型培养物中的尿路上皮细胞组织成多层上皮,从多孔膜向表面呈现终末分化。在I型和II型培养物的表面,表层尿路上皮细胞高度分化。它们形成紧密连接,并具有不对称单位膜斑块,这是膀胱表层细胞终末分化的标志。这些培养的表层细胞细胞角蛋白20也呈阳性。相反,III型培养物中的尿路上皮细胞显示出有限的存活能力,并且以低分化的单层形式生长。这些结果表明,固有层和尿路上皮细胞之间的相互细胞间信号传导对于在体外实现尿路上皮细胞的终末分化至关重要。

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