Stuart J, Gordon P A, Lee T R
Histochem J. 1975 Sep;7(5):471-87. doi: 10.1007/BF01003884.
Diseases of the blood and bone marrow are commonly associated with abnormalities of oxido-reductase and lysosomal enzymes within individual erythrocytes and leucocytes. There are considerable technical difficulties, however, in adapting enzyme histochemical techniques to the study of haemopoietic tissue since individual cells are readily disrupted during processing, show variable enzyme activity according to the stage of maturation, and possess a lipoprotein cytoplasmic membrane which hinders reagent penetration. Cytochemical techniques for the study of oxido-reductase systems are of importance in the study of the neutrophil in infected patients, the erythrocyte in glucose-6-phosphate dehydrogenase deficiency, and the primitive blast cell in acute leukaemia. Lysosomal enzymes are of importance in the study of the neutrophil in infected patients and in the differential diagnosis of acute leukaemia. Some examples of recent studies of these enzyme systems are given to illustrate technical procedures involving cytocentrifugation of cells on to glass slides, adjustment of the osmolality of the reaction mixture, and the study of smeared cells as opposed to cells incubated in suspension.
血液和骨髓疾病通常与单个红细胞和白细胞内氧化还原酶及溶酶体酶的异常有关。然而，将酶组织化学技术应用于造血组织研究存在相当大的技术困难，因为单个细胞在处理过程中容易被破坏，根据成熟阶段显示出可变的酶活性，并且具有阻碍试剂渗透的脂蛋白细胞质膜。用于研究氧化还原酶系统的细胞化学技术在感染患者的中性粒细胞、葡萄糖-6-磷酸脱氢酶缺乏症患者的红细胞以及急性白血病的原始母细胞研究中具有重要意义。溶酶体酶在感染患者的中性粒细胞研究和急性白血病的鉴别诊断中具有重要意义。给出了这些酶系统近期研究的一些例子，以说明涉及将细胞离心涂片到载玻片上、调整反应混合物的渗透压以及研究涂片细胞而非悬浮培养细胞的技术程序。
