Busch M, Thoma R, Schiller I, Corboz L, Pospischil A
Institutes of Veterinary Pathology and Veterinary Bacteriology, University of Zurich, Switzerland.
J Vet Med B Infect Dis Vet Public Health. 2000 Aug;47(6):471-80. doi: 10.1046/j.1439-0450.2000.00415.x.
The aim of this study was to investigate further the role of chlamydiae as pathogens in the genital tracts of sows at slaughter. Genital tracts of 101 randomly selected sows were collected and specimens of genital tract localizations were systematically examined for chlamydiae using immunohistochemistry and PCR. In the genital tracts of 10 sows, Chlamydia psittaci DNA was detected by PCR, and was further typed as 'serotype 1' in nine cases and as avian strain 6 BC in one animal. However, all specimens examined by immunohistochemistry were negative for chlamydiae. Pooled samples of scalding tank water were additionally investigated for 95 animals. Of these samples, 63.2% contained chlamydial DNA, mostly C. trachomatis, and in one sample C. psittaci 'serotype 1'. Although in most cases contamination through influx of faecally contaminated scalding water is a possible reason for the positive PCR results in the genital tract, latent infection cannot be excluded. In conclusion, the results obtained suggest that chlamydiae are of no or only minor importance in the examined group of Swiss breeding sows. Nevertheless, the role and significance of chlamydiae as pathogens in porcine reproductive disorders remain unresolved and require further investigation.
本研究的目的是进一步调查衣原体作为屠宰母猪生殖道病原体的作用。收集了101头随机选择的母猪的生殖道，并使用免疫组织化学和聚合酶链反应（PCR）对生殖道定位标本进行衣原体系统检测。在10头母猪的生殖道中，通过PCR检测到鹦鹉热衣原体DNA，其中9例进一步分型为“血清型1”，1例为禽株6 BC。然而，所有通过免疫组织化学检查的标本衣原体均为阴性。另外对95头母猪的烫毛槽水混合样本进行了调查。在这些样本中，63.2%含有衣原体DNA，主要是沙眼衣原体，1个样本中含有鹦鹉热衣原体“血清型1”。尽管在大多数情况下，经粪便污染的烫毛水流入造成污染可能是生殖道PCR结果呈阳性的原因，但不能排除潜伏感染。总之，所得结果表明，衣原体在所检查的瑞士繁殖母猪组中无重要影响或影响很小。然而，衣原体作为猪生殖障碍病原体的作用和意义仍未明确，需要进一步研究。
