Eastes W, Potter R M, Hadley J G
Owens Corning, Science and Technology Center, 2790 Columbus Road, Route 16, Granville, OH 43023-1200, USA.
Inhal Toxicol. 2000 Nov;12(11):1037-54. doi: 10.1080/08958370050164644.
Although the dissolution rate of a fiber was originally defined by a measurement of dissolution in simulated lung fluid in vitro, it is feasible to determine it from animal studies as well. The dissolution rate constant for a fiber may be extracted from the decrease in long fiber diameter observed in certain intratracheal instillation experiments or from the observed long fiber retention in short-term biopersistence studies. These in vivo dissolution rates agree well with those measured in vitro for the same fibers. For those special types of fibers, the high-alumina rock wool fibers that could not be measured in vitro, the method provides a way of obtaining a chemical dissolution rate constant from an animal study. The inverse of the in vivo dissolution rate, the fiber dissolution time, correlates well with the weighted half life of long fibers in a biopersistence study, and the in vivo dissolution rate may be estimated accurately from this weighted half-life.
尽管纤维的溶解速率最初是通过在体外模拟肺液中的溶解测量来定义的，但从动物研究中确定它也是可行的。纤维的溶解速率常数可以从某些气管内滴注实验中观察到的长纤维直径的减小，或从短期生物持久性研究中观察到的长纤维保留情况中提取。这些体内溶解速率与相同纤维的体外测量结果非常吻合。对于那些无法在体外测量的特殊类型的纤维，即高铝岩棉纤维，该方法提供了一种从动物研究中获得化学溶解速率常数的途径。体内溶解速率的倒数，即纤维溶解时间，与生物持久性研究中长纤维的加权半衰期密切相关，并且可以从这个加权半衰期准确估计体内溶解速率。
