一种用于检测血管紧张素II 1型受体A1166C多态性的基于MADGE凝胶的高通量MS-PCR方法。
A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.
作者信息
Hunt C C, Burley J E, Chapman C M, Beilby J P
机构信息
The Western Australian Centre for Pathology and Medical Research (PathCentre), Perth, Australia.
出版信息
Physiol Genomics. 1999 Aug 31;1(2):71-3. doi: 10.1152/physiolgenomics.1999.1.2.71.
We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT(1)) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT(1) A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.
我们开发了一种用于测定血管紧张素 II 1 型受体（AT(1)）A1166C 基因多态性的高精度、低成本、单步、诱变分离聚合酶链反应（MS-PCR）方法。使用微量滴定阵列对角线凝胶电泳（MADGE）系统确定基因型。我们将 MS-PCR 方法与等位基因特异性寡核苷酸杂交和 Dde I 消化技术进行了比较，以确定 AT(1) A1166C 基因型。MS-PCR 和 MADGE 的结合为大规模人群研究中的高通量单核苷酸多态性基因分型提供了一个模型。
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