Davies J B, Basáñez M G, Frontado H, Villamizar N, Raybould J N, Petralanda I
Liverpool School of Tropical Medicine, UK.
Med Vet Entomol. 2000 Sep;14(3):321-31. doi: 10.1046/j.1365-2915.2000.00220.x.
Phosphoglucomutase (PGM) and trehalase (Tre) isoenzymes of five species of Simulium blackflies (Diptera: Simuliidae), vectors of onchocerciasis in Venezuela, were investigated by means of a portable electrophoresis field kit. Tre differed between S. incrustatum and S. oyapockense s.l. Electrophoretic variation of Tre in other members of the S. amazonicum and S. incrustatum groups merit further investigation. PGM appears to be more useful for separating populations within species complexes. Multiple populations and/or seasonal changes in population structure of S. guianense s.l., S. exiguum s.l. and S. metallicum s.l. were inferred from elecrophoretic variation of PGM.
利用便携式电泳场试剂盒，对委内瑞拉盘尾丝虫病传播媒介——五种蚋属蚋（双翅目：蚋科）的磷酸葡萄糖变位酶（PGM）和海藻糖酶（Tre）同工酶进行了研究。在包壳蚋和奥亚波克蚋复合体之间，海藻糖酶存在差异。亚马逊蚋组和包壳蚋组其他成员中海藻糖酶的电泳变异值得进一步研究。磷酸葡萄糖变位酶似乎在区分物种复合体中的种群方面更有用。从磷酸葡萄糖变位酶的电泳变异推断出圭亚那蚋复合体、微小蚋复合体和金属蚋复合体的多个种群和/或种群结构的季节性变化。
