Liu Y, Nakamura T, Yamamoto Y, Matsumoto K, Sekine T, Ueda H, Shimizu Y
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Japan.
ASAIO J. 2000 Sep-Oct;46(5):536-9. doi: 10.1097/00002480-200009000-00005.
The aim of this study was to reduce the antigenicity of tracheal allografts by detergent treatment. We attempted to apply these grafts to tracheal immunosuppressant-free allotransplantation. Fresh tracheal grafts were harvested from donor beagle dogs and treated with a detergent at 4 degrees C for 48 hours. By using treated grafts, we have performed tracheal immunosuppressant-free transplantation in six dogs at an intrathoracic five-ring defect. Implanted grafts were covered with an omental pedicle. In five of the six grafts, complete removal of the epithelium and mixed glands was recognized with both light microscopy and scanning electron microscopy. The appearance of the cartilage cells in the grafts was similar to those in fresh trachea. Five dogs that received detergent treated grafts survived uneventfully. The grafts had been incorporated by the host trachea without stenosis. On the one tracheal graft in which removal of the epithelium was incomplete (noted after implantation), moderate stenosis occurred 1 month after placement. These results suggest that removal of the tracheal epithelium and mixed glands can remarkably reduce tracheal antigenicity. A tracheal graft that has its epithelium and mixed glands removed can be used in tracheal immunosuppressant-free allotransplantation.
本研究的目的是通过去污剂处理降低气管同种异体移植物的抗原性。我们试图将这些移植物应用于无气管免疫抑制剂的同种异体移植。从供体比格犬获取新鲜气管移植物，并在4℃下用去污剂处理48小时。通过使用处理过的移植物，我们在6只犬的胸内五环缺损处进行了无气管免疫抑制剂的移植。植入的移植物用网膜蒂覆盖。在6个移植物中的5个中，通过光学显微镜和扫描电子显微镜均确认上皮和混合腺完全去除。移植物中软骨细胞的外观与新鲜气管中的相似。接受去污剂处理移植物的5只犬顺利存活。移植物已被宿主气管整合且无狭窄。在1个上皮去除不完全的气管移植物中（植入后发现），放置1个月后出现中度狭窄。这些结果表明，去除气管上皮和混合腺可显著降低气管抗原性。去除上皮和混合腺的气管移植物可用于无气管免疫抑制剂的同种异体移植。
