Salman H, Bergman M, Bessler H, Alexandrova S, Djaldetti M
Department of Medicine C, Rabin Medical Center, Golda Campus, Petah Tiqva, Israel.
Cryobiology. 2000 Aug;41(1):66-71. doi: 10.1006/cryo.2000.2267.
Hypothermia affects various components of the immune system, leading to impaired immune resistance. To examine the in vitro effect of low temperature on the ultrastructure and phagocytic function of rat peritoneal macrophages, cells were incubated at 4, 10, 24, and 37 degrees C for 60 min. Subsequently, their ultrastructure and capacity to engulf latex particles and generate superoxide anions were evaluated. The results showed a close inverse relationship between incubation temperature and ultrastructural changes, i.e., the lower the temperature, the higher the number of altered cells. In addition, at lower temperatures the number of cells capable of phagocytosis was reduced; the cells engulfed fewer particles per cell and generated less superoxide anions. These findings may be relevant for explaining the increased susceptibility to bacterial infections under hypothermic conditions.
体温过低会影响免疫系统的各个组成部分，导致免疫抵抗力受损。为了研究低温对大鼠腹腔巨噬细胞超微结构和吞噬功能的体外影响，将细胞分别在4、10、24和37摄氏度下孵育60分钟。随后，评估它们的超微结构以及吞噬乳胶颗粒和产生超氧阴离子的能力。结果显示孵育温度与超微结构变化之间存在密切的负相关关系，即温度越低，细胞改变的数量越多。此外，在较低温度下，能够进行吞噬作用的细胞数量减少；每个细胞吞噬的颗粒减少，产生的超氧阴离子也减少。这些发现可能有助于解释低温条件下细菌感染易感性增加的原因。
