Liacopoulos-Briot M, Lambert F, Halpern B
Ann Immunol (Paris). 1975 Apr;126(3):249-65.
A specific anti-macrophage serum (AMS) has been obtained by immunizing rabbits with mouse peritoneal exudate cells and after repeated absorption with mouse erythrocytes, thymocytes and splenic lymphocytes. A comparative study of the cytotoxic activities of this serum on macrophages, thymocytes and lymph node cells, before and after absorption, showed that macrophage posses antigens which are common to both thymic and lymph node lymphocytes, and other antigens which are in common with lymph node lymphocytes but absent on thymocytes, as well as antigens being particular to macrophages. The absorbed sera had a definite cytotoxic effect on macrophages in vitro. This effect was observed when complement was added immediately or 4 hours after incubation with serum. It was lost after 24 hours. An inhibitory effects of AMS on phagocytosis in vivo has also been observed, which disappeared after one day. Antibody production in vivo was only moderately affected by a pretreatment with AMS. However, addition of AMS to spleen cell cultures stimulated with sheep red blood cells inhibited the appearance of hemolytic plaque forming cells.
通过用小鼠腹腔渗出细胞免疫兔子，并先后用小鼠红细胞、胸腺细胞和脾淋巴细胞反复吸收，获得了一种特异性抗巨噬细胞血清（AMS）。对该血清在吸收前后对巨噬细胞、胸腺细胞和淋巴结细胞的细胞毒活性进行的比较研究表明，巨噬细胞具有胸腺淋巴细胞和淋巴结淋巴细胞共有的抗原，以及与淋巴结淋巴细胞共有的但胸腺细胞所没有的其他抗原，还有巨噬细胞特有的抗原。吸收后的血清在体外对巨噬细胞有明确的细胞毒作用。当补体在与血清孵育后立即或4小时加入时可观察到这种作用。24小时后该作用消失。还观察到AMS对体内吞噬作用有抑制作用，该作用在一天后消失。体内抗体产生仅受到AMS预处理的适度影响。然而，向用绵羊红细胞刺激的脾细胞培养物中加入AMS会抑制溶血空斑形成细胞的出现。
