Ishihama A
National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka 411-8540, Japan.
Annu Rev Microbiol. 2000;54:499-518. doi: 10.1146/annurev.micro.54.1.499.
The promoter recognition specificity of Escherichia coli RNA polymerase is modulated by replacement of the sigma subunit in the first step and by interaction with transcription factors in the second step. The overall differentiated state of approximately 2000 molecules of the RNA polymerase in a single cell can be estimated after measurement of both the intracellular concentrations and the RNA polymerase-binding affinities for all seven species of the sigma subunit and 100-150 transcription factors. The anticipated impact from this line of systematic approach is that the prediction of the expression hierarchy of approximately 4000 genes on the E. coli genome can be estimated.
大肠杆菌RNA聚合酶的启动子识别特异性在第一步通过替换σ亚基以及在第二步通过与转录因子相互作用来调节。在测量了细胞内浓度以及RNA聚合酶与所有七种σ亚基和100 - 150种转录因子的结合亲和力之后，可以估计单个细胞中大约2000个RNA聚合酶分子的整体分化状态。这种系统方法预期的影响是，可以估计大肠杆菌基因组上大约4000个基因的表达层次。
