Nakanishi H
Aust N Z J Surg. 1975 Aug;45(3):309-14. doi: 10.1111/j.1445-2197.1975.tb05217.x.
A study was made of the healing and revascularization of intestinal anastomoses in the small and large intestines of rabbits. Single-layer and double-layer suture, techniques were used and compared, and revascularization of the anastomoses was particularly examined after devascularization of their distal segments. The anastomotic segments were removed at intervals after construction varying from one to six weeks and studied histologically after haemotoxylin and eosin staining, by xylol-clear thick sections and by angiography, a gelatin Micropaque emulsion being used to fill the vascular network. Forty-four rabbit intestinal anastomoses were studied. Inverted intestinal anastomoses took about two weeks to develop continuous mucosa on the luminal side, and were completely healed after six weeks. Revascularization began after eight days with both single-layer and double-layer anastomoses, proceeding from the submucosal and the surrounding muscular layers. The vascular pattern mirrored the healing stage of the anastomosis. Irregular fine vessels crossing the anastomoses were seen in specimens examined at the second week. After three or four weeks, the vascular patterns of the anastomoses showed an irregularity of calibre and direction because of the presence of residual scar tissue, and the mucosal surfaces were covered with regenerating epithelium. Healing and revascularization were complete after six weeks.
对兔小肠和大肠肠吻合口的愈合及血管再生进行了一项研究。采用单层和双层缝合技术并进行比较，尤其在吻合口远段去血管化后检查吻合口的血管再生情况。在构建吻合口后1至6周的不同时间间隔切除吻合段，经苏木精和伊红染色、二甲苯透明厚切片以及血管造影（使用明胶微球混悬液填充血管网络）后进行组织学研究。共研究了44个兔肠吻合口。倒置肠吻合口在管腔侧形成连续黏膜约需两周时间，六周后完全愈合。单层和双层吻合口在八天后均开始血管再生，从黏膜下层和周围肌层开始。血管模式反映了吻合口的愈合阶段。在第二周检查的标本中可见穿过吻合口的不规则细血管。三或四周后，由于残留瘢痕组织的存在，吻合口的血管模式显示管径和方向不规则，黏膜表面覆盖着再生上皮。六周后愈合和血管再生完成。
