Cook J T, Day S, Dunn P P, Welsh K I, Bunce M
Nuffield Department of Surgery, Oxford Transplant Centre, Churchill Hospital, United Kingdom.
Tissue Antigens. 2000 Aug;56(2):178-9. doi: 10.1034/j.1399-0039.2000.560213.x.
We have identified a new HLA-B39 allele through polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing of exons 2 and 3. This novel allele was identified in three HLA-identical siblings of Turkish origin. This allele only differs from HLA-B3903 at a unique single nucleotide substitution (T for C) at position 365 in exon 3 which results in an amino acid change in codon 98 of methionine (ATG) to threonine (ACG). The sequencing enabled the development of a monospecific PCR-SSP reaction which can be used to discriminate between HLA-B3924 and other B39 alleles.
我们通过使用序列特异性引物（SSP）的聚合酶链反应（PCR）以及外显子2和3的序列分型鉴定出一个新的HLA - B39等位基因。这个新等位基因是在三名土耳其裔HLA相同的同胞中鉴定出来的。该等位基因仅与HLA - B3903在外显子3第365位有一个独特的单核苷酸替换（C替换为T），这导致密码子98处的氨基酸由甲硫氨酸（ATG）变为苏氨酸（ACG）。测序使得能够开发一种单特异性PCR - SSP反应，可用于区分HLA - B3924和其他B39等位基因。
