Keech D R, Ramadan H, Mathers P
Department of Otolaryngology-Head and Neck Surgery and Biochemistry, West Virginia University, Morgantown, WV, USA.
Otolaryngol Head Neck Surg. 2000 Oct;123(4):363-7. doi: 10.1067/mhn.2000.108204.
Rhinosinusitis is a common disease affecting an estimated 14% of the population. Although there is general agreement in the literature regarding acute rhinosinusitis, chronic rhinosinusitis is not as well studied, and no consensus has been reached regarding the bacterial etiology. The goal of this study was to test chronic rhinosinusitis mucosal specimens using the polymerase chain reaction (PCR) for aerobic bacterial content and to compare the results with standard culture data.
Routine culture samples grew 50% aerobic bacteria, whereas PCR detected 62% aerobic bacteria contamination.
PCR detected more bacteria in mucosal samples than in standard culture, but standard culture of this mucosa reflects the general aerobic bacteria found in chronic rhinosinusitis, with no predominant species of aerobic bacteria.
The analysis of chronic rhinosinusitis mucosa with the PCR method should give a more accurate picture of the bacteria found in chronic rhinosinusitis so that proper therapy can be instituted.
鼻窦炎是一种常见疾病，估计影响14%的人口。尽管文献中对急性鼻窦炎已达成普遍共识，但慢性鼻窦炎的研究尚不充分，关于其细菌病因也未达成共识。本研究的目的是使用聚合酶链反应（PCR）检测慢性鼻窦炎黏膜标本中的需氧菌含量，并将结果与标准培养数据进行比较。
常规培养样本中50%培养出需氧菌，而PCR检测到62%的需氧菌污染。
PCR检测到黏膜样本中的细菌比标准培养更多，但这种黏膜的标准培养反映了慢性鼻窦炎中常见的需氧菌，没有优势需氧菌种。
用PCR方法分析慢性鼻窦炎黏膜应能更准确地呈现慢性鼻窦炎中发现的细菌情况，从而制定恰当的治疗方案。
