Sellergren B, Andersson L I
Department of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, Mainz, Duesbergweg 10-14, Mainz, 55099, Germany.
Methods. 2000 Sep;22(1):92-106. doi: 10.1006/meth.2000.1040.
The first part of the review describes a method for the synthesis of molecularly imprinted polymers for use in binding assays. The method considers the many factors involved that affect the recognition properties of the materials and describes an approach to screening and optimization of these factors. The second part describes the development of binding assays using such polymers. This includes the use of different labels, the effect of solvent and buffer, the scale of the assay (amount of solid polymer), and how these influence the quality of the assay in terms of sensitivity, selectivity, and speed of analysis.
综述的第一部分描述了一种用于结合测定的分子印迹聚合物的合成方法。该方法考虑了影响材料识别特性的诸多因素，并描述了一种筛选和优化这些因素的方法。第二部分描述了使用此类聚合物进行结合测定的进展。这包括不同标记物的使用、溶剂和缓冲液的影响、测定规模（固体聚合物的量），以及这些因素如何在灵敏度、选择性和分析速度方面影响测定质量。
