Zhang Z, Wu Y, Cheng W, Wu R
Department of Pharmacy, Henan Medical University, Zhengzhou, 450052, China.
Clin Chim Acta. 2000 Nov;301(1-2):205-11. doi: 10.1016/s0009-8981(00)00310-7.
Mutations of K-ras gene play an important role in neoplastic progression. The capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) technique is available for the detection of gene mutations. Using an automated capillary electrophoresis with short-chain linear polyacrylamide, after denaturation of PCR products, injections were performed at reverse polarity of 5 kV for 15 s and the separations were carried out under a constant voltage of 8 kV. Of 16 specimens of lung cancer tissue, two specimens were found to have abnormal peaks in the electrophoretogram. CE-SSCP is rapid, automated, and has high performance.
K-ras基因的突变在肿瘤进展中起重要作用。毛细管电泳-单链构象多态性（CE-SSCP）技术可用于检测基因突变。使用配备短链线性聚丙烯酰胺的自动毛细管电泳，PCR产物变性后，以5 kV的反向极性进样15秒，并在8 kV的恒定电压下进行分离。在16份肺癌组织标本中，有两份标本在电泳图中出现异常峰。CE-SSCP快速、自动化且性能高。
