Bekku S, Mochizuki H, Yamamoto T, Ueno H, Takayama E, Tadakuma T
Department of Surgery I, National Defense Medical College, Saitama, Japan.
Hepatogastroenterology. 2000 Jul-Aug;47(34):998-1001.
BACKGROUND/AIMS: This study was undertaken to elucidate the correlation between the expression of carbonic anhydrase I or II and the characteristic features of colorectal cancer.
The carbonic anhydrase I or II expressions of 74 colorectal cancer patients were analyzed by Western blotting. The relative intensity of cancer to the paired normal mucosa was calculated, and then compared with the clinicopathological parameters. Furthermore, a multivariate analysis for synchronous distant metastasis was undertaken.
The expression of carbonic anhydrase I in colon cancer or carbonic anhydrase II in rectal cancer with Duke's D was found to be significantly lower than that with Duke's B or C, respectively. Similarly, carbonic anhydrase I in colon cancer or carbonic anhydrase II in rectal cancer with moderate-severe budding was found to be significantly lower than that with none-mild budding, respectively. Based on the findings of a logistic regression analysis, carbonic anhydrase I was adopted for colon cancer (P = 0.057) and carbonic anhydrase II for rectal cancer (P = 0.008) regarding synchronous distant metastasis.
The expressions of carbonic anhydrase I and II correlated with biological aggressiveness of colorectal cancer and synchronous distant metastasis, especially carbonic anhydrase I for colon cancer and carbonic anhydrase II for rectal cancer.
背景/目的：本研究旨在阐明碳酸酐酶I或II的表达与结直肠癌特征之间的相关性。
采用蛋白质免疫印迹法分析74例结直肠癌患者的碳酸酐酶I或II表达情况。计算癌组织与配对正常黏膜的相对强度，然后与临床病理参数进行比较。此外，对同时性远处转移进行多因素分析。
发现Duke's D期结肠癌中碳酸酐酶I的表达或直肠癌中碳酸酐酶II的表达分别显著低于Duke's B期或C期。同样，中度-重度芽生的结肠癌中碳酸酐酶I或直肠癌中碳酸酐酶II的表达分别显著低于无-轻度芽生的情况。基于逻辑回归分析结果，就同时性远处转移而言，结肠癌采用碳酸酐酶I（P = 0.057），直肠癌采用碳酸酐酶II（P = 0.008）。
碳酸酐酶I和II的表达与结直肠癌的生物学侵袭性及同时性远处转移相关，尤其是结肠癌中的碳酸酐酶I和直肠癌中的碳酸酐酶II。
