缺乏糖蛋白gp85的爱泼斯坦-巴尔病毒无法感染B细胞和上皮细胞。

Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and epithelial cells.

作者信息

Oda T, Imai S, Chiba S, Takada K

机构信息

Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15 W7, Kita-ku, Sapporo, 060-8638, Japan.

出版信息

Virology. 2000 Oct 10;276(1):52-8. doi: 10.1006/viro.2000.0531.

DOI:10.1006/viro.2000.0531

PMID:11021994

Abstract

To evaluate whether the BXLF2 gene of Epstein-Barr virus (EBV), which codes gp85 protein, is essential for infection of B cells and epithelial cells, we analyzed the infectivity of an EBV recombinant lacking gp85. The cells that were infected with the BXLF2-disrupted virus were unable to express gp85 proteins that could be detected by mouse monoclonal antibody E1D1, specific for gp85/gp25 complexes. The BXLF2-disrupted EBV had the ability to attach to, but not infect, B cells. On the other hand, the same virus failed to bind to and infect NU-GC-3, a human gastric adenocarcinoma cell line that is susceptible to EBV infection. The results indicate that the gp85 is used for infection of not only B cells but also epithelial cells and suggest that the gp85 is necessary for attaching the virus to epithelial cells.

摘要

为了评估编码gp85蛋白的爱泼斯坦-巴尔病毒（EBV）的BXLF2基因对于B细胞和上皮细胞感染是否至关重要，我们分析了一种缺乏gp85的EBV重组体的感染性。感染了BXLF2缺失病毒的细胞无法表达可被针对gp85/gp25复合物的小鼠单克隆抗体E1D1检测到的gp85蛋白。BXLF2缺失的EBV具有附着于B细胞但不感染B细胞的能力。另一方面，同一病毒无法结合并感染对EBV感染敏感的人胃腺癌细胞系NU-GC-3。结果表明，gp85不仅用于B细胞的感染，也用于上皮细胞的感染，提示gp85对于病毒附着于上皮细胞是必需的。