Gwozdz J M, Thompson K G, Murray A, West D M, Manktelow B W
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
Aust Vet J. 2000 Sep;78(9):622-4. doi: 10.1111/j.1751-0813.2000.tb11938.x.
To evaluate a polymerase chain reaction-based assay for the detection of Mycobacterium avium subsp paratuberculosis in blood and liver biopsies from subclinically infected sheep.
A direct PCR assay for the detection of M a paratuberculosis was applied to liver biopsy specimens and to samples of blood that were sequentially collected over 53 weeks from 14 sheep infected experimentally with the organism.
Of 117 blood samples from the 14 experimentally infected sheep, two tested positive in the PCR assay. Both positive results were obtained in two subclinically infected sheep that had paratuberculosis later confirmed by histological examination at necropsy. However, the assay failed to detect the target DNA in samples of blood from five other sheep with histologically confirmed paratuberculosis. Similarly, the PCR assay on liver biopsy specimens collected 32 weeks after administration of M a paratuberculosis gave only two positive results, both of which were obtained in sheep with histological evidence of paratuberculosis.
The PCR assay on blood and liver biopsies does not provide a useful method for the diagnosis of M a paratuberculosis infection in subclinically infected sheep.
评估一种基于聚合酶链反应的检测方法，用于检测亚临床感染绵羊血液和肝脏活检组织中的副结核分枝杆菌。
将一种用于检测副结核分枝杆菌的直接聚合酶链反应检测方法应用于肝脏活检标本以及从14只经该病原体实验性感染的绵羊身上在53周内连续采集的血液样本。
在这14只经实验感染的绵羊的117份血液样本中，两份在聚合酶链反应检测中呈阳性。这两个阳性结果均来自两只亚临床感染绵羊，其副结核病后来在尸检时经组织学检查得到证实。然而，该检测方法未能在另外五只经组织学证实患有副结核病的绵羊的血液样本中检测到目标DNA。同样，在接种副结核分枝杆菌32周后采集的肝脏活检标本上进行的聚合酶链反应检测仅得到两个阳性结果，且均来自有副结核病组织学证据的绵羊。
对血液和肝脏活检组织进行聚合酶链反应检测，并非诊断亚临床感染绵羊副结核分枝杆菌感染的有效方法。
