采用高效液相色谱法和紫外分光光度法测定片剂中的佐匹克隆。

Determination of zopiclone in tablets by HPLC and UV-spectrophotometry.

作者信息

Paw B, Misztal G

机构信息

Department of Medicinal Chemistry, Faculty of Pharmacy, Medical Academy, Lublin, Poland.

出版信息

J Pharm Biomed Anal. 2000 Oct;23(5):819-23. doi: 10.1016/s0731-7085(00)00379-4.

DOI:10.1016/s0731-7085(00)00379-4

PMID:11022907

Abstract

Rapid, simple and accurate chromatographic (HPLC) and spectrophotometric methods for the determination of zopiclone in tablets were elaborated. Acetonitrile was found to be a suitable extraction solvent. The samples were chromatographed on Nova-Pak C18 column and UV detection at 304 nm. The elution was achieved isocratically with a mobile phase of 0.067 M phosphate buffer pH 7.95 - acetonitrile (55:45, v/v). Diazepam was applied as an internal standard. The method was validated for precision, linearity, accuracy and limit of detection. The recovery (mean +/- SD) in HPLC was 99.85 + 0.04% and in the UV-spectrophotometry 100.08 +/- 0.09%.

摘要

建立了快速、简便且准确的色谱法（高效液相色谱法）和分光光度法用于测定片剂中的佐匹克隆。发现乙腈是合适的萃取溶剂。样品在Nova-Pak C18柱上进行色谱分离，并在304 nm处进行紫外检测。采用0.067 M pH 7.95的磷酸盐缓冲液-乙腈（55:45，v/v）的流动相进行等度洗脱。地西泮用作内标。该方法针对精密度、线性、准确度和检测限进行了验证。高效液相色谱法的回收率（平均值±标准差）为99.85 + 0.04%，紫外分光光度法的回收率为100.08 ± 0.09%。