Walters H, Smits W, van Kessel J, van Os G
Eur J Biochem. 1975 Oct 15;58(2):269-72. doi: 10.1111/j.1432-1033.1975.tb02372.x.
The dissociation and association behaviour of 70-S ribosomes of Escherichia coli has been studied. It has been shown that the dissociation-association reaction can be both a real dynamic equilibrium and a non-equilibrium reaction, dependent upon the ionic conditions of the solvent. At relatively high ionic strength (I = 0.15 M or more) the dissociation-association reaction is an equilibrium reaction, whereas at lower ionic strength (I = 0.1 M or less) there is no dynamic equilibrium between 70-S ribosomes and its subunits. In the latter case a hysteresis in the dissociation-association reaction is observed. Whether there is a dynamic equilibrium or not can be demonstrated by a single centrifugation experiment, using the analytical ultracentrifuge.
对大肠杆菌70-S核糖体的解离和缔合行为进行了研究。结果表明，解离-缔合反应既可以是真正的动态平衡反应，也可以是非平衡反应，这取决于溶剂的离子条件。在相对较高的离子强度（I = 0.15M或更高）下，解离-缔合反应是平衡反应，而在较低的离子强度（I = 0.1M或更低）下，70-S核糖体与其亚基之间不存在动态平衡。在后一种情况下，观察到解离-缔合反应中的滞后现象。是否存在动态平衡可以通过使用分析超速离心机进行的单次离心实验来证明。
