Bachellerie J P, Puvion E, Zalta J P
Eur J Biochem. 1975 Oct 15;58(2):327-37. doi: 10.1111/j.1432-1033.1975.tb02379.x.
Extranucleolar elements were isolated from mammalian cell nuclei and characterized by correlative biochemical-ultrastructural studies. These complex structures were shown to retain a supra-particle arrangement closely resembling the organization in situ of definite nuclear areas. In particular the morphological RNA - protein species, namely perichromatin fibrils and granules, were detected in association with characteristic regions of chromatin. By autoradiographic experiments, we were able to show that perichromatin fibrils represent the morphological state of newly formed heterogeneous nuclear RNA (hnRNA). Chromatin - RNA - protein complexes were further fractionated by means of a treatment dissociating the chromatin component. A minor part of DNA, possibly involved in the biosynthesis and the processing of hnRNA, remains linked to the resulting RNA - protein network.
从哺乳动物细胞核中分离出核仁外成分，并通过相关的生化超微结构研究对其进行表征。这些复杂结构显示保留了一种超颗粒排列，与特定核区域的原位组织非常相似。特别是，在染色质的特征区域检测到了形态学上的RNA - 蛋白质种类，即染色质周纤维和颗粒。通过放射自显影实验，我们能够证明染色质周纤维代表新形成的不均一核RNA（hnRNA）的形态状态。通过解离染色质成分的处理，进一步分离染色质 - RNA - 蛋白质复合物。一小部分可能参与hnRNA生物合成和加工的DNA仍与所得的RNA - 蛋白质网络相连。
