鉴定幽门螺杆菌在酸性条件下生长所必需的基因座。
Identification of loci essential for the growth of Helicobacter pylori under acidic conditions.
作者信息
Bijlsma J J, Lie-A-Ling M, Nootenboom I C, Vandenbroucke-Grauls C M, Kusters J G
机构信息
Department of Medical Microbiology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
J Infect Dis. 2000 Nov;182(5):1566-9. doi: 10.1086/315855. Epub 2000 Oct 9.
To persist in the hostile acidic environment of the stomach, Helicobacter pylori must survive acid shock and grow at acidic pH. Of a library of 1250 random mutants screened for isolates unable to grow at low pH, 10 mutants were detected that were unable to grow at pH 4.8. However, all 10 mutants were resistant to acid shock. Four mutants had an insertion in genes of unknown function. One mutant was affected in lepA, an orthologue of a membrane GTPase. Three mutants were disrupted in loci involved in the transport of H(+) ions or other cations (FRaseI, czcA, and aldo-keto reductase). Two mutants were affected in loci that contribute to acid resistance in other microorganisms (uvrA and atpF'). Thus, at least 10 loci not related to urease are essential for the growth of H. pylori under acidic conditions and should be critical for lifelong infection by this pathogen.
为了在胃部充满敌意的酸性环境中存活，幽门螺杆菌必须经受住酸休克并在酸性pH值下生长。在对1250个随机突变体文库进行筛选，以找出无法在低pH值下生长的分离株时，检测到10个突变体无法在pH 4.8下生长。然而，所有10个突变体都对酸休克具有抗性。四个突变体在功能未知的基因中存在插入。一个突变体在lepA（一种膜GTP酶的直系同源物）中受到影响。三个突变体在参与H⁺离子或其他阳离子转运的基因座（FRaseI、czcA和醛糖酮还原酶）中被破坏。两个突变体在对其他微生物的耐酸性有贡献的基因座（uvrA和atpF'）中受到影响。因此，至少10个与脲酶无关的基因座对于幽门螺杆菌在酸性条件下的生长至关重要，并且对于该病原体的终身感染应该至关重要。
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