Lass-Flörl C, Rath P, Niederwieser D, Kofler G, Würzner R, Krezy A, Dierich M P
Institute of Hygiene, University of Innsbruck, Austria.
J Hosp Infect. 2000 Sep;46(1):31-5. doi: 10.1053/jhin.2000.0799.
During a three-year period nine patients with haematological diseases after myeloablative chemotherapy died from invasive fungal infections caused by Aspergillus terreus. The hospital inanimate environment was monitored and A. terreus was cultured from potted plants in the vicinity of the patients. The patients (N = 14) and the environmental isolates (N = 2) were fingerprinted by RAPD-PCR with four different primers. Based on RAPD patterns the patients' isolates were differentiated into five different types; the environmental isolates represented two types. The isolates of four patients were identical to those found in the environment. Five additional patients were infected by RAPD types not found in the environment. One patient was infected with two different types. The data indicate a hospital-acquired infection in many of the patients and underline the need for careful environmental monitoring of units in which high-risk patients are housed.
在三年期间，9例接受清髓性化疗后患有血液系统疾病的患者死于土曲霉引起的侵袭性真菌感染。对医院的无生命环境进行了监测，在患者附近的盆栽植物中培养出了土曲霉。使用四种不同引物通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）对14例患者和2株环境分离株进行指纹图谱分析。根据RAPD图谱，患者的分离株被分为五种不同类型；环境分离株代表两种类型。4例患者的分离株与在环境中发现的分离株相同。另外5例患者感染了环境中未发现的RAPD类型。1例患者感染了两种不同类型。数据表明许多患者发生了医院获得性感染，并强调了对收治高危患者的病房进行仔细环境监测的必要性。
