Glockling S L, Beakes G W
Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne, NE1 7RU, United Kingdom.
J Invertebr Pathol. 2000 Oct;76(3):208-15. doi: 10.1006/jipa.2000.4967.
Recently fired gun cells of Haptoglossa heteromorpha, an aplanosporic nematode parasite, were examined ultrastructurally. The everted tubes of the fired cells had penetrated the cuticle of a nematode, and infective sporidia were developing inside the host body. The nematode cuticle was penetrated by the narrow, walled part of the tube below the needle chamber. The lower unwalled part of the tube tail formed the sporidium. The developing sporidium had a multilayered fibrous outer coating and the plasma membrane was separated from the wall in places. Sporidia contained biphasic membrane-bound vesicles that had been generated by the Golgi dictyosome during gun cell development. Immediately following gun cell firing, the nuclear envelope of the sporidium nucleus was not apparent, and the sporidium nucleus contained clusters of electron-dense particles concentrated in the nucleolar region. We compare the structures and organelles found in the mature gun cell with those in the fired cell and attempt to identify the membranous layers around the sporidium.
最近，对一种无隔孢子线虫寄生虫异形粘孢菌（Haptoglossa heteromorpha）刚射出的枪细胞进行了超微结构检查。射出细胞的外翻管穿透了线虫的角质层，感染性孢子正在宿主体内发育。线虫角质层被针状腔下方管子狭窄的有壁部分穿透。管子尾部下方无壁的下部形成了孢子。发育中的孢子有一层多层纤维状外层，质膜在某些地方与壁分离。孢子含有双相膜结合囊泡，这些囊泡是在枪细胞发育过程中由高尔基体生成的。枪细胞射出后，孢子核的核膜不明显，孢子核含有集中在核仁区域的电子致密颗粒簇。我们将成熟枪细胞中发现的结构和细胞器与射出细胞中的进行比较，并试图识别孢子周围的膜层。
