Rieu J P, Upadhyaya A, Glazier J A, Ouchi N B, Sawada Y
Département de Physique des Matériaux, Université Claude Bernard, Lyon I, 69622 Villeurbanne Cedex, France.
Biophys J. 2000 Oct;79(4):1903-14. doi: 10.1016/S0006-3495(00)76440-X.
Cell motion within cellular aggregates consists of both random and coherent components. We used confocal microscopy to study the center of mass displacements and deformations of single endodermal Hydra cells in two kinds of cellular aggregates, ectodermal and endodermal. We first carefully characterize the center of mass displacements using standard statistical analysis. In both aggregates, cells perform a persistent random walk, with the diffusion constant smaller in the more cohesive endodermal aggregate. We show that a simple parametric method is able to describe cell deformations and relate them to displacements. These deformations are random, with their amplitude and direction uncorrelated with the center of mass motion. Unlike for an isolated cell on a substrate, the random forces exerted by the surrounding cells predominate over the deformation of the cell itself, causing the displacements of a cell within an aggregate.
细胞聚集体内的细胞运动由随机成分和相干成分组成。我们使用共聚焦显微镜研究了两种细胞聚集体（外胚层和内胚层）中单个内胚层水螅细胞的质心位移和变形。我们首先使用标准统计分析仔细表征质心位移。在这两种聚集体中，细胞都进行持续的随机游走，在凝聚力更强的内胚层聚集体中扩散常数较小。我们表明，一种简单的参数方法能够描述细胞变形并将它们与位移联系起来。这些变形是随机的，其幅度和方向与质心运动不相关。与基质上的单个细胞不同，周围细胞施加的随机力在细胞自身变形中占主导地位，导致聚集体内细胞的位移。
