2b 同种型免疫球蛋白及其 F(ab) 和 F(c) 片段的展开/变性
The unfolding/denaturation of immunogammaglobulin of isotype 2b and its F(ab) and F(c) fragments.
作者信息
Vermeer A W, Norde W, van Amerongen A
机构信息
Laboratory for Physical Chemistry and Colloid Science, Wageningen University, 6703 HB Wageningen, The Netherlands.
出版信息
Biophys J. 2000 Oct;79(4):2150-4. doi: 10.1016/S0006-3495(00)76462-9.
Abstract
The unfolding and further denaturation of IgG and its F(ab) and F(c) fragments were studied both on a macroscopic and molecular level, using differential scanning calorimetry and circular dichroism spectroscopy, respectively. It was shown that the structural integrity of the F(ab) and F(c) units was retained after fragmentation of the IgG. The F(ab) fragment denatured at approximately 61 degrees C and the F(c) fragment at 71 degrees C. The structural transitions observed in the whole IgG is the sum effect of those determined for the isolated F(ab) and F(c) fragments.
摘要
分别使用差示扫描量热法和圆二色光谱法，在宏观和分子水平上研究了IgG及其F(ab)和F(c)片段的展开和进一步变性。结果表明，IgG片段化后，F(ab)和F(c)单元的结构完整性得以保留。F(ab)片段在约61℃变性，F(c)片段在71℃变性。在整个IgG中观察到的结构转变是分离的F(ab)和F(c)片段所确定的结构转变的总和效应。
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本文引用的文献
1
CD Spectroscopy of Proteins Adsorbed at Flat Hydrophilic Quartz and Hydrophobic Teflon Surfaces.吸附在亲水性平面石英和疏水性聚四氟乙烯表面的蛋白质的圆二色光谱。
J Colloid Interface Sci. 2000 May 15;225(2):394-397. doi: 10.1006/jcis.2000.6769.
2
The thermal stability of immunoglobulin: unfolding and aggregation of a multi-domain protein.免疫球蛋白的热稳定性：一种多结构域蛋白的解折叠与聚集
Biophys J. 2000 Jan;78(1):394-404. doi: 10.1016/S0006-3495(00)76602-1.
3
Structural changes of IgG induced by heat treatment and by adsorption onto a hydrophobic Teflon surface studied by circular dichroism spectroscopy.通过圆二色光谱法研究热处理以及吸附到疏水性聚四氟乙烯表面所诱导的IgG结构变化。
Biochim Biophys Acta. 1998 Sep 16;1425(1):1-12. doi: 10.1016/s0304-4165(98)00048-8.
4
Multistep denaturation and hierarchy of disulfide bond cleavage of a monoclonal antibody.单克隆抗体的多步变性及二硫键断裂的层次结构
Anal Biochem. 1997 May 1;247(2):247-56. doi: 10.1006/abio.1997.2062.
5
Fc receptors and their interactions with immunoglobulins.Fc受体及其与免疫球蛋白的相互作用。
Annu Rev Cell Dev Biol. 1996;12:181-220. doi: 10.1146/annurev.cellbio.12.1.181.
6
Signal transduction by Fc receptors: the Fc epsilon RI case.Fc受体介导的信号转导：以FcεRI为例
Immunol Today. 1993 May;14(5):222-6. doi: 10.1016/0167-5699(93)90167-j.
7
Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.人IgG Fc受体的异质性：分子层面与临床意义
Immunol Today. 1993 May;14(5):215-21. doi: 10.1016/0167-5699(93)90166-I.
8
Anatomy of the antibody molecule.抗体分子的剖析。
Mol Immunol. 1994 Feb;31(3):169-217. doi: 10.1016/0161-5890(94)90001-9.
9
Crystal structure of the complex of rat neonatal Fc receptor with Fc.大鼠新生Fc受体与Fc复合物的晶体结构
Nature. 1994 Nov 24;372(6504):379-83. doi: 10.1038/372379a0.
10
Binding of monomeric immunoglobulin G triggers Fc gamma RI-mediated endocytosis.单体免疫球蛋白G的结合触发FcγRI介导的内吞作用。
J Biol Chem. 1994 Sep 30;269(39):24396-402.
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