反映非理想大分子缔合的沉降平衡分布分析。
Analysis of sedimentation equilibrium distributions reflecting nonideal macromolecular associations.
作者信息
Wills P R, Jacobsen M P, Winzor D J
机构信息
Department of Physics, University of Auckland, Auckland, New Zealand.
出版信息
Biophys J. 2000 Oct;79(4):2178-87. doi: 10.1016/S0006-3495(00)76466-6.
Abstract
A rigorous statistical-mechanical approach is adopted to derive general quantitative expressions that allow for the effects of thermodynamic nonideality in equilibrium measurements reflecting interaction between dissimilar macromolecular reactants. An analytical procedure based on these expressions is then formulated for obtaining global estimates of equilibrium constants and the corresponding reference thermodynamic activities of the free reactants in each of several sedimentation equilibrium experiments. The method is demonstrated by application to results from an ultracentrifugal study of an electrostatic interaction between ovalbumin and cytochrome c (Winzor, D. J., M. P. Jacobsen, and P. R. Wills. 1998. Biochemistry. 37:2226-2233). It is demonstrated that reliable estimates of relevant thermodynamic parameters are extracted from the data through statistical analysis by means of a simple nonlinear fitting procedure.
摘要
采用一种严格的统计力学方法来推导通用的定量表达式，这些表达式考虑了热力学非理想性在反映不同大分子反应物之间相互作用的平衡测量中的影响。然后基于这些表达式制定一种分析程序，以在几个沉降平衡实验中的每一个中获得平衡常数的全局估计值以及游离反应物相应的参考热力学活度。通过将该方法应用于对卵清蛋白和细胞色素c之间静电相互作用的超速离心研究结果（Winzor, D. J., M. P. Jacobsen, and P. R. Wills. 1998. Biochemistry. 37:2226 - 2233）来证明该方法。结果表明，通过简单的非线性拟合程序进行统计分析，可以从数据中提取相关热力学参数的可靠估计值。
相似文献
1
Analysis of sedimentation equilibrium distributions reflecting nonideal macromolecular associations.反映非理想大分子缔合的沉降平衡分布分析。
Biophys J. 2000 Oct;79(4):2178-87. doi: 10.1016/S0006-3495(00)76466-6.
2
Direct analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.
Biochemistry. 1998 Feb 24;37(8):2226-33. doi: 10.1021/bi972211v.
3
Direct analysis of sedimentation equilibrium distributions reflecting complex formation between cytochrome c and ovalbumin.
Biochem Soc Trans. 1998 Nov;26(4):741-5. doi: 10.1042/bst0260741.
4
An ultracentrifugal approach to quantitative characterization of the molecular assembly of a physiological electron-transfer complex: the interaction of electron-transferring flavoprotein with trimethylamine dehydrogenase.一种用于定量表征生理电子传递复合物分子组装的超速离心方法：电子传递黄素蛋白与三甲胺脱氢酶的相互作用
Eur J Biochem. 1997 Jan 15;243(1-2):393-9. doi: 10.1111/j.1432-1033.1997.0393a.x.
5
Effect of sucrose on the dimerization of alpha-chymotrypsin. Allowance for thermodynamic nonideality arising from the presence of a small inert solute.蔗糖对α-糜蛋白酶二聚化的影响。考虑到由少量惰性溶质的存在而产生的热力学非理想性。
Biophys Chem. 1988 Sep;31(3):287-94. doi: 10.1016/0301-4622(88)80034-6.
6
Sedimentation equilibrium of protein solutions in concentrated guanidinium chloride. Thermodynamic nonideality and protein heterogeneity.蛋白质溶液在浓氯化胍中的沉降平衡。热力学非理想性与蛋白质异质性。
Biochemistry. 1972 Feb 29;11(5):687-97. doi: 10.1021/bi00755a004.
7
Analyses of thermodynamic data for concentrated hemoglobin solutions using scaled particle theory: implications for a simple two-state model of water in thermodynamic analyses of crowding in vitro and in vivo.使用标度粒子理论对浓缩血红蛋白溶液的热力学数据进行分析：对体外和体内拥挤现象热力学分析中简单双态水模型的启示。
Biophys J. 1995 Mar;68(3):835-46. doi: 10.1016/S0006-3495(95)80260-2.
8
Thermodynamic nonideality in macromolecular solutions: interpretation of virial coefficients.大分子溶液中的热力学非理想性：维里系数的解释。
Arch Biochem Biophys. 1993 Jan;300(1):206-12. doi: 10.1006/abbi.1993.1029.
9
COVOL: an interactive program for evaluating second virial coefficients from the triaxial shape or dimensions of rigid macromolecules.COVOL：一个用于根据刚性大分子的三轴形状或尺寸评估第二维里系数的交互式程序。
Biophys J. 1999 May;76(5):2432-8. doi: 10.1016/S0006-3495(99)77398-4.
10
Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation.使用Lamm方程的数值解对非相互作用和自缔合溶质进行沉降分析。
Biophys J. 1998 Sep;75(3):1503-12. doi: 10.1016/S0006-3495(98)74069-X.
引用本文的文献
1
Measuring Ultra-Weak Protein Self-Association by Non-ideal Sedimentation Velocity.通过非理想沉降速度测量超弱蛋白自组装。
J Am Chem Soc. 2019 Feb 20;141(7):2990-2996. doi: 10.1021/jacs.8b11371. Epub 2019 Feb 6.
2
Foreword to 'Quantitative and analytical relations in biochemistry'-a special issue in honour of Donald J. Winzor's 80th birthday.《生物化学中的定量与分析关系》前言——纪念唐纳德·J·温佐尔80岁生日的特刊
Biophys Rev. 2016 Dec;8(4):269-277. doi: 10.1007/s12551-016-0227-5. Epub 2016 Nov 4.
3
A Hilly path through the thermodynamics and statistical mechanics of protein solutions.一条穿越蛋白质溶液热力学与统计力学的崎岖之路。
Biophys Rev. 2016 Dec;8(4):291-298. doi: 10.1007/s12551-016-0226-6. Epub 2016 Oct 25.
4
Comparison of methods for characterizing nonideal solute self-association by sedimentation equilibrium.通过沉降平衡表征非理想溶质自缔合的方法比较
Biophys J. 2009 Aug 5;97(3):886-96. doi: 10.1016/j.bpj.2009.05.028.
5
Second virial coefficients as a measure of protein--osmolyte interactions.作为蛋白质与渗透溶质相互作用量度的第二维里系数
Protein Sci. 2001 Jan;10(1):12-6. doi: 10.1110/ps.29301.
本文引用的文献
1
Interpretation of thermodynamic non-ideality in sedimentation equilibrium experiments on proteins.蛋白质沉降平衡实验中热力学非理想性的解读。
Biophys Chem. 2000 May 15;84(3):217-25. doi: 10.1016/s0301-4622(00)00124-1.
2
Direct analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.
Biochemistry. 1998 Feb 24;37(8):2226-33. doi: 10.1021/bi972211v.
3
The effect of self-association on the interaction of the Escherichia coli regulatory protein TyrR with DNA.自我缔合对大肠杆菌调节蛋白TyrR与DNA相互作用的影响。
J Mol Biol. 1996 Nov 15;263(5):671-84. doi: 10.1006/jmbi.1996.0607.
4
Thermodynamic analysis of the effects of small inert cosolutes in the ultracentrifugation of noninteracting proteins.非相互作用蛋白质超速离心中小的惰性共溶质效应的热力学分析
Biochemistry. 1996 Oct 8;35(40):13173-9. doi: 10.1021/bi960939q.
5
5-hydroxytryptophan as a new intrinsic probe for investigating protein-DNA interactions by analytical ultracentrifugation. Study of the effect of DNA on self-assembly of the bacteriophage lambda cI repressor.5-羟色氨酸作为一种新型内在探针，用于通过分析超速离心研究蛋白质与DNA的相互作用。DNA对噬菌体λ cI阻遏蛋白自组装影响的研究。
Biochemistry. 1993 Mar 16;32(10):2469-72. doi: 10.1021/bi00061a003.
6
Interaction of the DNA-binding domain of Drosophila heat shock factor with its cognate DNA site: a thermodynamic analysis using analytical ultracentrifugation.果蝇热休克因子的DNA结合结构域与其同源DNA位点的相互作用：利用分析型超速离心进行的热力学分析
Protein Sci. 1994 Jul;3(7):1040-51. doi: 10.1002/pro.5560030706.
7
Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.采用非线性最小二乘法技术对分析型超速离心机的数据进行分析。
Biophys J. 1981 Dec;36(3):575-88. doi: 10.1016/S0006-3495(81)84753-4.
8
The indefinite self-association of lysozyme: consideration of composition-dependent activity coefficients.溶菌酶的不确定自缔合：对组成依赖性活度系数的考量
Biophys Chem. 1980 Feb;11(1):71-82. doi: 10.1016/0301-4622(80)85009-5.
9
Exponential analysis of concentration or concentration difference data for discrete molecular weight distributions in sedimentation equilibrium.沉降平衡中离散分子量分布的浓度或浓度差数据的指数分析。
Biochemistry. 1970 Jan 20;9(2):435-45. doi: 10.1021/bi00804a035.
10
The direct analysis of sedimentation equilibrium results obtained with polymerizing systems.
Biophys Chem. 1975 Apr;3(2):169-76. doi: 10.1016/0301-4622(75)80007-x.
Zotero 插件