限制表达可延长通过逆转录病毒导入动物体内的外源基因的表达时间。
Restricting expression prolongs expression of foreign genes introduced into animals by retroviruses.
作者信息
Pinto V B, Prasad S, Yewdell J, Bennink J, Hughes S H
机构信息
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
出版信息
J Virol. 2000 Nov;74(21):10202-6. doi: 10.1128/jvi.74.21.10202-10206.2000.
Abstract
If foreign genes are ubiquitously expressed in mice using a viral vector, expression is abrogated by CD8(+) cells in 2 to 4 weeks. However, if the expression of the genes is confined to skeletal muscle cells, the CD8(+) T-cell response is much weaker and expression is maintained for more than 6 weeks. These data show that restricting the expression of foreign genes to skeletal muscle cells and presumably to other cells that are inefficient at antigen presentation can prolong the expression of a foreign gene product.
摘要
如果使用病毒载体使外源基因在小鼠中普遍表达，那么在2至4周内，CD8(+)细胞会消除这种表达。然而，如果基因的表达局限于骨骼肌细胞，CD8(+) T细胞反应则要弱得多，并且表达可以维持超过6周。这些数据表明，将外源基因的表达限制在骨骼肌细胞以及可能在抗原呈递效率低下的其他细胞中，可以延长外源基因产物的表达时间。
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