Craig T L, Denlinger D L
The Ohio State University, Department of Entomology, Columbus, OH 43210-1220, USA.
Gene. 2000 Sep 19;255(2):381-8. doi: 10.1016/s0378-1119(00)00307-3.
We have isolated and sequenced a 1308bp clone from a pupal brain cDNA library of the flesh fly, Sarcophaga crassipalpis, showing 97% amino acid (aa) sequence similarity to Ceratitis capitata 60S acidic ribosomal protein P0 (CcP0) and 93% aa sequence similiarity to Drosophila melanogaster P0 (DmP0). DmP0 is a multifunctional protein necessary for efficient protein translation of the 60S ribosome as well as DNA repair via AP3 endonuclease activity. In this study, we observed that S. crassipalpis P0 (ScP0) is cyclically regulated throughout the fly's overwintering pupal diapause. Expression of ScP0 cycles out of phase with the 4day cycles of O(2) consumption: the peak day of O(2) consumption is characterized by low ScP0 expression, while high expression is noted during the trough of the O(2) consumption cycle. The O(2) cycles, which are in turn driven by cycles of juvenile hormone (JH), can be eliminated by application of a JH analog (JHA). Pupae rendered acyclic with a JHA application consume O(2) at a constant high rate and ScP0 is consistently downregulated. Our findings thus suggest that the cyclic nature of ScP0 regulation during pupal diapause is linked to the JH-mediated metabolic cycles characteristic of this species.
我们从肉蝇（Sarcophaga crassipalpis）蛹脑cDNA文库中分离并测序了一个1308bp的克隆，其氨基酸（aa）序列与地中海实蝇（Ceratitis capitata）60S酸性核糖体蛋白P0（CcP0）的相似性为97%，与黑腹果蝇（Drosophila melanogaster）P0（DmP0）的aa序列相似性为93%。DmP0是一种多功能蛋白，对于60S核糖体的高效蛋白质翻译以及通过AP3核酸内切酶活性进行DNA修复是必需的。在本研究中，我们观察到肉蝇P0（ScP0）在果蝇越冬蛹滞育期间受到周期性调控。ScP0的表达与氧气消耗的4天周期不同步：氧气消耗的峰值日以ScP0低表达为特征，而在氧气消耗周期的低谷期则观察到高表达。反过来由保幼激素（JH）周期驱动的氧气周期，可以通过应用JH类似物（JHA）来消除。用JHA处理使蛹失去周期性，其以恒定的高速率消耗氧气，并且ScP0持续下调。因此，我们的研究结果表明，蛹滞育期间ScP0调控的周期性与该物种特有的JH介导的代谢周期有关。
