Lehoux D E, Levesque R C
Microbiologie Moléculaire et Génie des Protéines, Pavillon Charles-Eugène Marchand et Faculté de Médecine, Université Laval, Sainte-Foy, G1K 7P4, Québec, Canada.
Curr Opin Biotechnol. 2000 Oct;11(5):434-9. doi: 10.1016/s0958-1669(00)00124-5.
Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.
现在，签名标签诱变（STM）技术有了多种变体，该方法可应用于大多数在宿主系统中具有STM可选择表型的病原体。对来自11种细菌的15040个突变体进行STM筛选，鉴定出323个体内减毒突变体。作为一种基因组扫描工具，STM将产生关于功能未知基因的信息以及对于理解微生物致病机制至关重要的信息。
