Duprat A M, Romanovsky A, Hurychová D, Machá J
J Embryol Exp Morphol. 1975 Aug;34(1):113-23.
Differentiating myoblasts from urodele amphibians were cultivated in vitro and treated by immunofluorescence techniques and (or) inhibitors of RNA and protein syntheses. These experiments lead to the conclusion that the differentiation of these cells is directed by long-lived m-RNAs which are already present in morphologically undifferentiated cells and may act for at least 6 days. Contrary to observations on cultivated myogenic cells from other groups, the fusion of urodele myoblasts is not a prerequisite for a normal and functional differentiation (contractions) since isolated myoblasts differentiate quite normally. The appearance and further evolution of myosin and actin occur synchronously in this biological system.
对来自有尾两栖动物的成肌细胞进行体外培养，并通过免疫荧光技术和（或）RNA及蛋白质合成抑制剂进行处理。这些实验得出的结论是，这些细胞的分化由长寿命mRNA指导，这些mRNA已存在于形态未分化的细胞中，并且可能至少发挥作用6天。与对来自其他组的培养肌原性细胞的观察结果相反，有尾两栖动物成肌细胞的融合不是正常和功能性分化（收缩）的先决条件，因为分离的成肌细胞分化得相当正常。在这个生物系统中，肌球蛋白和肌动蛋白的出现及进一步演变是同步发生的。
