Nishikawa J, Saito K, Sasaki M, Tomigahara Y, Nishihara T
Laboratory of Environmental Biochemistry, School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka, 565, Japan.
Biochem Biophys Res Commun. 2000 Oct 14;277(1):209-15. doi: 10.1006/bbrc.2000.3649.
Benzoate X receptor (BXR) is a member of the nuclear hormone receptor superfamily and is activated by alkyl esters of amino benzoic acids and expressed during early development stages. We report here a second BXR, which was cloned by screening Xenopus laevis embryonic cDNA libraries. This new BXR, termed BXRbeta, exhibits 97% identity in the DNA-binding domain and 79% identity in the ligand-binding domain in amino acid sequence to previously reported Xenopus BXR. The BXRbeta strongly binds as a heterodimer with retinoid X receptor (RXR) to direct repeats of an AGGTCA motif spaced 4 or 5 base pairs apart and activates transcription by addition of methyprednisolone and dexamethasone as well as amino- and hydroxybenzoates.
苯甲酸酯X受体（BXR）是核激素受体超家族的成员，可被氨基苯甲酸的烷基酯激活，并在早期发育阶段表达。我们在此报告第二种BXR，它是通过筛选非洲爪蟾胚胎cDNA文库克隆得到的。这种新的BXR，称为BXRβ，在DNA结合域的氨基酸序列中与先前报道的非洲爪蟾BXR具有97%的同一性，在配体结合域具有79%的同一性。BXRβ作为异二聚体与视黄酸X受体（RXR）强烈结合，以结合间隔4或5个碱基对的AGGTCA基序的直接重复序列，并通过添加甲基强的松龙、地塞米松以及氨基和羟基苯甲酸来激活转录。
