Okusa Y, Ichikura T, Mochizuki H, Shinomiya N
Department of Surgery I, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Int J Oncol. 2000 Nov;17(5):1001-5. doi: 10.3892/ijo.17.5.1001.
To assess the role of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) on the invasive potential of cancer cells, in vitro experiments were performed using two human gastric cancer cell lines, NUGC-3 and MKN-28. NUGC-3 cells secreted a higher level of uPA than MKN-28 cells, while the uPAR expression of NUGC-3 cells was lower than that of MKN-28 cells. Both cancer cell lines expressed Met protein and did not express hepatocyte growth factor (HGF). In Matrigel invasion assay, MKN-28 cells demonstrated significantly lower invasion index than NUGC-3 cells. The addition of exogenous uPA significantly increased the invasive activity of MKN-28 cells. The uPA expression in NUGC-3 cells was enhanced by adding conditioned media of fibroblast cells or HGF. These results suggest that uPA promotes the invasive capacity of the uPAR-positive cancer cells, and that stromal cells may play an important role in cancer cell invasion by supplying uPA and/or promoting uPA production.
为评估尿激酶型纤溶酶原激活剂（uPA）和uPA受体（uPAR）在癌细胞侵袭潜能中的作用，使用两种人胃癌细胞系NUGC-3和MKN-28进行了体外实验。NUGC-3细胞分泌的uPA水平高于MKN-28细胞，而NUGC-3细胞的uPAR表达低于MKN-28细胞。两种癌细胞系均表达Met蛋白，不表达肝细胞生长因子（HGF）。在基质胶侵袭实验中，MKN-28细胞的侵袭指数显著低于NUGC-3细胞。添加外源性uPA显著增加了MKN-28细胞的侵袭活性。通过添加成纤维细胞条件培养基或HGF可增强NUGC-3细胞中的uPA表达。这些结果表明，uPA促进uPAR阳性癌细胞的侵袭能力，并且基质细胞可能通过提供uPA和/或促进uPA产生在癌细胞侵袭中发挥重要作用。
